Calculators
Oligo properties calculator
Enter a DNA or RNA sequence to calculate the properties you need for design and handling. The same thermodynamic modelling runs, at greater depth, inside Discovery AI.
Your sequence
Enter a sequence
Only valid bases are counted (A, C, G, T). Spaces and numbers are ignored.
Results
Calculated properties
Length
21 nt
GC content
28.6%
Melting temperature (Tm)
44.6 °C
GC estimate (Marmur–Doty style)
Molecular weight
6437.3 g/mol
Anhydrous, single strand
Extinction coefficient
221,200
L / (mol·cm) at 260 nm, nearest neighbour
nmol per OD₂₆₀
4.5
≈ 29.1 µg per OD
Base composition: A:9 C:3 G:3 T:6
Values are estimates for handling and design. Tm depends on strand and salt concentration; Discovery AI uses full nearest neighbour thermodynamics.
Design and order in one workflow
Take your sequence into Discovery AI to model chemistry and off target risk, then order synthesis ready oligos.
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